Generation of a heterozygous C-peptide-mCherry reporter human iPSC line (HMGUi001-A-8)

The peptide hormone insulin produced by pancreatic β-cells undergoes post-transcriptional processing before secretion. In particular, C-peptide is cleaved from pro-insulin to generate mature insulin. Here, we introduce a C-peptide-mCherry human iPSC line (HMGUi001-A-8). was generated CRISPR/Cas9 mediated heterozygous insertion of the mCherry sequence into exon 3 locus. We demonstrate that pluripotent and efficiently differentiates towards β-like cells, which localize red fluorescent fusion protein in containing granules. Hence, HMGUi001-A-8 valuable resource purify derived cells follow insulin-containing granules real time.